atm antibody 2c1 Search Results


atm  (Novus Biologicals)
93
Novus Biologicals atm
Figure 1. ATRIP promotes the association of ATR and TopBP1. (A) Nuclear extracts from 293T cells treated with 8 Gy of IR or mock-treated were incubated with equal amounts of recombinant GST-tagged fragments of TopBP1 fragments ([AAD] amino acids 978–1286 [Kumagai et al. 2006]; [7&8] BRCT repeats 7 and 8, amino acids 1182–1522; [AAD + 7&8] amino acids 978–1522) bound to glutathione beads. Proteins bound to the beads were eluted, separated by SDS-PAGE, and immunoblotted <t>with</t> <t>antibodies</t> to ATR, ATRIP, or <t>ATM</t> (WB). A duplicate gel was stained with Coomassie blue to verify equal amounts of GST-tagged TopBP1 proteins (CB). (B) Nuclear ex- tracts from 293T cells transfected with a vector encoding ATR or vectors encoding ATR and ATRIP were incubated with re- combinant fragments of TopBP1 bound to glutathione beads. Bound proteins were eluted, separated by SDS-PAGE, and im- munoblotted with an antibody to ATR. (C) Nuclear extracts from U2OS cells stably expressing HA-tagged wild-type (wt) ATRIP or HA-tagged ATRIP lacking the C-terminal 32 amino acids (C) were incubated with recombinant GST-tagged frag- ments of TopBP1 bound to glutathione beads. Proteins bound to the beads were eluted, separated by SDS-PAGE, and blotted with an anti-HA antibody. Input in all experiments is 5% of the extract added to the binding reaction.
Atm, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/atm+antibody+2c1/ATM+Antibody+(2C1)/pm18519640-277-5-6
Average 93 stars, based on 1 article reviews
atm - by Bioz Stars, 2026-10
93/100 stars
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anti atm  (Novus Biologicals)
93
Novus Biologicals anti atm
Figure 1. ATRIP promotes the association of ATR and TopBP1. (A) Nuclear extracts from 293T cells treated with 8 Gy of IR or mock-treated were incubated with equal amounts of recombinant GST-tagged fragments of TopBP1 fragments ([AAD] amino acids 978–1286 [Kumagai et al. 2006]; [7&8] BRCT repeats 7 and 8, amino acids 1182–1522; [AAD + 7&8] amino acids 978–1522) bound to glutathione beads. Proteins bound to the beads were eluted, separated by SDS-PAGE, and immunoblotted <t>with</t> <t>antibodies</t> to ATR, ATRIP, or <t>ATM</t> (WB). A duplicate gel was stained with Coomassie blue to verify equal amounts of GST-tagged TopBP1 proteins (CB). (B) Nuclear ex- tracts from 293T cells transfected with a vector encoding ATR or vectors encoding ATR and ATRIP were incubated with re- combinant fragments of TopBP1 bound to glutathione beads. Bound proteins were eluted, separated by SDS-PAGE, and im- munoblotted with an antibody to ATR. (C) Nuclear extracts from U2OS cells stably expressing HA-tagged wild-type (wt) ATRIP or HA-tagged ATRIP lacking the C-terminal 32 amino acids (C) were incubated with recombinant GST-tagged frag- ments of TopBP1 bound to glutathione beads. Proteins bound to the beads were eluted, separated by SDS-PAGE, and blotted with an anti-HA antibody. Input in all experiments is 5% of the extract added to the binding reaction.
Anti Atm, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/atm+antibody+2c1/ATM+Antibody+(2C1)/pmc04113274-42-9-10
Average 93 stars, based on 1 article reviews
anti atm - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier
atm antibody 2c1  (GeneTex)
90
GeneTex atm antibody 2c1
Figure 1. ATRIP promotes the association of ATR and TopBP1. (A) Nuclear extracts from 293T cells treated with 8 Gy of IR or mock-treated were incubated with equal amounts of recombinant GST-tagged fragments of TopBP1 fragments ([AAD] amino acids 978–1286 [Kumagai et al. 2006]; [7&8] BRCT repeats 7 and 8, amino acids 1182–1522; [AAD + 7&8] amino acids 978–1522) bound to glutathione beads. Proteins bound to the beads were eluted, separated by SDS-PAGE, and immunoblotted <t>with</t> <t>antibodies</t> to ATR, ATRIP, or <t>ATM</t> (WB). A duplicate gel was stained with Coomassie blue to verify equal amounts of GST-tagged TopBP1 proteins (CB). (B) Nuclear ex- tracts from 293T cells transfected with a vector encoding ATR or vectors encoding ATR and ATRIP were incubated with re- combinant fragments of TopBP1 bound to glutathione beads. Bound proteins were eluted, separated by SDS-PAGE, and im- munoblotted with an antibody to ATR. (C) Nuclear extracts from U2OS cells stably expressing HA-tagged wild-type (wt) ATRIP or HA-tagged ATRIP lacking the C-terminal 32 amino acids (C) were incubated with recombinant GST-tagged frag- ments of TopBP1 bound to glutathione beads. Proteins bound to the beads were eluted, separated by SDS-PAGE, and blotted with an anti-HA antibody. Input in all experiments is 5% of the extract added to the binding reaction.
Atm Antibody 2c1, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/atm+antibody+2c1/atm+2c1+antibody/pm20048340-359-6-8
Average 90 stars, based on 1 article reviews
atm antibody 2c1 - by Bioz Stars, 2026-10
90/100 stars
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atm antibody 2c1 alexa fluor« 488  (Novus Biologicals)
N/A
The ATM Antibody 2C1 Alexa Fluor« 488 from Novus Biologicals is a mouse monoclonal antibody to ATM This antibody reacts with human mouse rat monkey The ATM Antibody 2C1 Alexa Fluor« 488 has been validated
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atm antibody 2c1 dylight 680  (Novus Biologicals)
N/A
The ATM Antibody 2C1 DyLight 680 from Novus Biologicals is a mouse monoclonal antibody to ATM This antibody reacts with human mouse rat monkey The ATM Antibody 2C1 DyLight 680 has been validated for the
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atm antibody 2c1 dylight 488  (Novus Biologicals)
N/A
The ATM Antibody 2C1 DyLight 488 from Novus Biologicals is a mouse monoclonal antibody to ATM This antibody reacts with human mouse rat monkey The ATM Antibody 2C1 DyLight 488 has been validated for the
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atm antibody 2c1 dylight 405  (Novus Biologicals)
N/A
The ATM Antibody 2C1 DyLight 405 from Novus Biologicals is a mouse monoclonal antibody to ATM This antibody reacts with human mouse rat monkey The ATM Antibody 2C1 DyLight 405 has been validated for the
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atm antibody 2c1 alexa fluor« 647  (Novus Biologicals)
N/A
The ATM Antibody 2C1 Alexa Fluor« 647 from Novus Biologicals is a mouse monoclonal antibody to ATM This antibody reacts with human mouse rat monkey The ATM Antibody 2C1 Alexa Fluor« 647 has been validated
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atm antibody 2c1 dylight 350  (Novus Biologicals)
N/A
The ATM Antibody 2C1 DyLight 350 from Novus Biologicals is a mouse monoclonal antibody to ATM This antibody reacts with human mouse rat monkey The ATM Antibody 2C1 DyLight 350 has been validated for the
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atm antibody (2c1-rb)  (Novus Biologicals)
N/A
The ATM Antibody (2C1-RB) from Novus is a ATM antibody to ATM. This antibody reacts with Human, Mouse, Rat. The ATM antibody has been validated for the following applications: Western Blot.
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atm antibody 2c1 dylight 755  (Novus Biologicals)
N/A
The ATM Antibody 2C1 DyLight 755 from Novus Biologicals is a mouse monoclonal antibody to ATM This antibody reacts with human mouse rat monkey The ATM Antibody 2C1 DyLight 755 has been validated for the
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atm antibody 2c1 pe  (Novus Biologicals)
N/A
The ATM Antibody 2C1 PE from Novus Biologicals is a mouse monoclonal antibody to ATM This antibody reacts with human mouse rat monkey The ATM Antibody 2C1 PE has been validated for the following applications
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Image Search Results


Figure 1. ATRIP promotes the association of ATR and TopBP1. (A) Nuclear extracts from 293T cells treated with 8 Gy of IR or mock-treated were incubated with equal amounts of recombinant GST-tagged fragments of TopBP1 fragments ([AAD] amino acids 978–1286 [Kumagai et al. 2006]; [7&8] BRCT repeats 7 and 8, amino acids 1182–1522; [AAD + 7&8] amino acids 978–1522) bound to glutathione beads. Proteins bound to the beads were eluted, separated by SDS-PAGE, and immunoblotted with antibodies to ATR, ATRIP, or ATM (WB). A duplicate gel was stained with Coomassie blue to verify equal amounts of GST-tagged TopBP1 proteins (CB). (B) Nuclear ex- tracts from 293T cells transfected with a vector encoding ATR or vectors encoding ATR and ATRIP were incubated with re- combinant fragments of TopBP1 bound to glutathione beads. Bound proteins were eluted, separated by SDS-PAGE, and im- munoblotted with an antibody to ATR. (C) Nuclear extracts from U2OS cells stably expressing HA-tagged wild-type (wt) ATRIP or HA-tagged ATRIP lacking the C-terminal 32 amino acids (C) were incubated with recombinant GST-tagged frag- ments of TopBP1 bound to glutathione beads. Proteins bound to the beads were eluted, separated by SDS-PAGE, and blotted with an anti-HA antibody. Input in all experiments is 5% of the extract added to the binding reaction.

Journal: Genes & development

Article Title: TopBP1 activates ATR through ATRIP and a PIKK regulatory domain.

doi: 10.1101/gad.1666208

Figure Lengend Snippet: Figure 1. ATRIP promotes the association of ATR and TopBP1. (A) Nuclear extracts from 293T cells treated with 8 Gy of IR or mock-treated were incubated with equal amounts of recombinant GST-tagged fragments of TopBP1 fragments ([AAD] amino acids 978–1286 [Kumagai et al. 2006]; [7&8] BRCT repeats 7 and 8, amino acids 1182–1522; [AAD + 7&8] amino acids 978–1522) bound to glutathione beads. Proteins bound to the beads were eluted, separated by SDS-PAGE, and immunoblotted with antibodies to ATR, ATRIP, or ATM (WB). A duplicate gel was stained with Coomassie blue to verify equal amounts of GST-tagged TopBP1 proteins (CB). (B) Nuclear ex- tracts from 293T cells transfected with a vector encoding ATR or vectors encoding ATR and ATRIP were incubated with re- combinant fragments of TopBP1 bound to glutathione beads. Bound proteins were eluted, separated by SDS-PAGE, and im- munoblotted with an antibody to ATR. (C) Nuclear extracts from U2OS cells stably expressing HA-tagged wild-type (wt) ATRIP or HA-tagged ATRIP lacking the C-terminal 32 amino acids (C) were incubated with recombinant GST-tagged frag- ments of TopBP1 bound to glutathione beads. Proteins bound to the beads were eluted, separated by SDS-PAGE, and blotted with an anti-HA antibody. Input in all experiments is 5% of the extract added to the binding reaction.

Article Snippet: The following antibodies were purchased: ATM (Novus), ATR and Chk1 (Santa Cruz Biotechnologies), Chk1 P317 (Cell Signaling), DNA-PKcs (Serotec), DNA-PKcs pS2056 (Abcam), HA.11 and Myc9E10 (Covance), and Flag M2 (Sigma).

Techniques: Incubation, Recombinant, SDS Page, Staining, Transfection, Plasmid Preparation, Stable Transfection, Expressing, Binding Assay